Anti Nuclear Antibody (ANA) by ELISA Report Format

Pathology
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What is the ANA Test?

The Anti Nuclear Antibody (ANA) test detects autoantibodies that target components within the cell nucleus. It is a key screening test for autoimmune diseases, particularly systemic lupus erythematosus (SLE).

What is an ANA by ELISA Report Format?

An ANA by ELISA report provides a quantitative measurement of anti-nuclear antibodies in the blood using enzyme-linked immunosorbent assay (ELISA), helping identify autoimmune activity.

Tests Included in ANA by ELISA Report

Test

Description

ANA by ELISA

Detects and quantifies anti-nuclear antibodies using ELISA technique.

Key Parts of an ANA by ELISA Report

Key Part

Description

ANA Level

Measured value reported in units per milliliter (U/mL).

Reference Range

Indicates the threshold below which the result is considered negative.

Result Status

Categorized as Negative, Equivocal, or Positive based on the antibody level.

Clinical Notes

Describes associated symptoms such as fatigue, joint pain, or skin rash.

Interpretation

Clarifies the clinical relevance of the result in the context of autoimmune disease.

Normal Range for ANA by ELISA Report

Result

Reference Range

Negative

<20 U/mL

Equivocal

20–39 U/mL

Positive

≥40 U/mL

Parameters to Consider in ANA by ELISA Report

Parameter

Interpretation

ANA Titer

Higher levels suggest stronger autoimmune activity.

Equivocal Result

May require repeat testing or confirmation by IFA (Immunofluorescence Assay).

Positive ANA

Indicates presence of autoantibodies; further specific tests may be needed.

Symptoms Correlation

Important to interpret results alongside clinical signs and other autoimmune markers.

Guidelines for ANA by ELISA Report Format

Guideline

Description

Units Consistency

Report ANA levels in U/mL using validated cut-offs.

Testing Method

Clearly mention ELISA as the assay method.

Diagnostic Follow-Up

Recommend specific antibody panels (e.g., anti-dsDNA, anti-Sm) if ANA is positive.

What Do the Results Mean?

Result Type

Interpretation

ANA Negative

No evidence of significant autoimmune activity.

ANA Equivocal

Borderline result; may need further testing or monitoring.

ANA Positive

Suggests autoimmune activity; may be associated with SLE, Sjögren’s syndrome, or mixed connective tissue disease.

ANA by ELISA Report Interpretation

Test

Interpretation

ANA by ELISA

Screens for autoimmune disease by detecting anti-nuclear antibodies; further evaluation may be required based on result and symptoms.

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