Widal (Slide Method) Report Format

Pathology
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What is the Widal Test?

The Widal test is a diagnostic test used to detect the presence of antibodies (agglutinins) against Salmonella species, particularly Salmonella typhi, which causes typhoid fever. The slide method is a rapid qualitative test that detects agglutination of the antigen-antibody reaction.

What is a Widal (Slide Method) Report Format?

A Widal (Slide Method) report presents the result of the slide agglutination test, indicating the presence or absence of Salmonella antibodies in the patient’s serum. It is used to screen for acute typhoid fever and monitor the presence of antibodies.

Tests Included in Widal Report

Test

Description

Widal (Slide)

Detects agglutination of specific Salmonella antigens (O, H) in the patient’s serum.

Key Parts of a Widal Report

Key Part

Description

Sample Type

Serum (blood sample).

Test Method

Slide agglutination method with antigen suspension.

Result

Reported as Positive, Negative, or Indeterminate.

Interpretation

Provides clinical context based on agglutination pattern.

Reference Range for Widal Report (Slide Method)

Antigen

Titer Level (1:20)

O Antigen (Somatic)

Non-Reactive (no agglutination)

H Antigen (Flagellar)

Non-Reactive (no agglutination)

Parameters to Consider in Widal Report (Slide Method)

Parameter

Interpretation

Positive Result

Suggests recent or active Salmonella infection, typically typhoid fever.

Negative Result

No antibodies detected—likely no current or past infection.

Indeterminate Result

Weak agglutination observed—requires follow-up testing or confirmation with a higher dilution.

Agglutination Pattern

Agglutination in one or more antigens indicates specific antibody presence.

Guidelines for Widal (Slide Method) Report Format

Guideline

Description

Sensitivity

The slide test is semi-quantitative; titer level interpretation is less precise than tube agglutination.

Timing of Test

Best used within the first 7 days of fever onset; later tests may show positive results from past infections.

Follow-Up

Positive slide results should be confirmed with a tube agglutination test (standard Widal test) and clinical correlation.

What Do the Results Mean?

Result Type

Interpretation

Negative

No detectable antibodies to Salmonella—no evidence of typhoid fever or past infection.

Positive

Suggests active infection, likely Salmonella typhi—confirm with further tests such as blood culture.

Indeterminate

Weak or borderline agglutination—requires further testing, including titers or blood culture.

Widal (Slide Method) Report Interpretation

Test

Interpretation

Widal (Slide)

A rapid screening test for Salmonella infection; positive results indicate possible typhoid fever but require confirmatory testing with blood culture or further Widal testing (tube method).

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Structure of the DoraysLIS Report

pathology report

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Essential identifiers included in the report templates are:

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  • Specimen type and quality
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  • Test method
  • normal range based on gender
  • Interpretation/contextual remarks/recommended action
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